1. What is RCF?

RCF (Relative Centrifugal Force) is the measurement of the gravitational force applied during centrifugation. It provides a standardized way to compare centrifugation conditions across different centrifuges with varying rotor sizes.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

Where:

• \( RCF \) — Relative centrifugal force (g)
• \( r \) — Radius from center of rotation to sample (cm)
• \( RPM \) — Rotations per minute (rev/min)
• \( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula converts rotational speed (RPM) to gravitational force (g-force) based on the radius of the centrifuge rotor.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is essential for reproducible laboratory results, proper sample separation, and ensuring experimental consistency across different centrifugation equipment.

4. Using the Calculator

Tips: Enter RPM in revolutions per minute and radius in centimeters. Ensure both values are positive numbers for accurate calculation.

5. Frequently Asked Questions (FAQ)

Q1: Why is RCF more important than RPM?
A: RCF provides a standardized measure of centrifugal force that accounts for rotor size, making it more reliable for reproducing results across different centrifuges.

Q2: How do I measure the radius?
A: Measure from the center of the rotor to the bottom of the sample tube when it's in the horizontal position during centrifugation.

Q3: What are typical RCF values used in laboratories?
A: Typical ranges vary from 200-500g for gentle separations to 10,000-20,000g for pelleting cells and up to 100,000g for ultracentrifugation.

Q4: Can I convert RCF back to RPM?
A: Yes, the formula can be rearranged: \( RPM = \sqrt{\frac{RCF}{1.118 \times 10^{-5} \times r}} \)

Q5: Does temperature affect RCF calculation?
A: No, RCF calculation is independent of temperature, but temperature can affect sample viscosity and separation efficiency during centrifugation.