1. What is Centrifuge RPM and RCF?

RPM (Revolutions Per Minute) and RCF (Relative Centrifugal Force) are two ways to express centrifuge speed. RPM measures rotational speed, while RCF (measured in × g) represents the actual gravitational force applied to samples, which is more meaningful for experimental reproducibility.

2. How Does the Calculator Work?

The calculator uses standard centrifuge conversion formulas:

Where:

• \( RCF \) — Relative Centrifugal Force (× g)
• \( RPM \) — Revolutions Per Minute
• \( r \) — Radius in centimeters (cm)
• 1.118 — Conversion constant

Explanation: The relationship between RPM and RCF depends on the square of the rotational speed and the radius from the center of rotation.

3. Importance of Centrifuge Calculations

Details: Accurate conversion between RPM and RCF is crucial for experimental reproducibility, protocol standardization, and ensuring consistent separation results across different centrifuge models and laboratories.

4. Using the Calculator

Tips: Select calculation type (RCF to RPM or RPM to RCF), enter the required values, and provide the radius in centimeters. Ensure all values are positive and radius is measured from the center of rotation to the sample.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of RPM?
A: RCF provides the actual force applied to samples, making results comparable across different centrifuges with varying rotor sizes.

Q2: How do I measure the radius?
A: Measure from the center of the centrifuge rotor to the bottom of the sample tube when placed in the rotor.

Q3: Are these formulas accurate for all centrifuges?
A: These formulas work for most standard laboratory centrifuges. Ultracentrifuges may have additional considerations.

Q4: What's the typical radius range?
A: Most laboratory centrifuges have radii between 5-20 cm, depending on the rotor type and model.

Q5: Can I use this for clinical centrifuges?
A: Yes, these formulas apply to both research and clinical centrifuges when proper radius measurements are taken.