1. What is Relative Centrifugal Force?

Relative Centrifugal Force (RCF) is the measurement of the force applied to a sample within a centrifuge. It is expressed in multiples of the earth's gravitational force (g) and provides a standardized way to compare centrifugation protocols across different equipment.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

Where:

• \( RCF \) — Relative centrifugal force (g-force, unitless)
• \( r \) — Radius from center of rotation to sample (cm)
• \( RPM \) — Revolutions per minute

Explanation: The formula converts rotational speed (RPM) into the actual gravitational force experienced by the sample, accounting for the centrifuge's radius.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is crucial for reproducible laboratory results, proper sample separation, and ensuring experimental consistency across different centrifuges and laboratories.

4. Using the Calculator

Tips: Enter the radius in centimeters (measure from center of rotor to sample) and RPM value. Both values must be positive numbers for accurate calculation.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of RPM?
A: RCF provides a standardized measure of centrifugal force that accounts for different centrifuge sizes, allowing for reproducible results across various equipment.

Q2: How do I measure the radius accurately?
A: Measure from the center of the centrifuge rotor to the middle of the sample tube when it's in the spinning position.

Q3: What are typical RCF values used in laboratories?
A: RCF values range from 100-20,000 g depending on the application - low for cell culture, medium for blood separation, high for molecular biology.

Q4: Can I convert RCF back to RPM?
A: Yes, the formula can be rearranged: \( RPM = \sqrt{\frac{RCF}{1.118 \times 10^{-5} \times r}} \)

Q5: Does temperature affect RCF calculation?
A: No, RCF calculation is purely mechanical. However, temperature affects sample viscosity and separation efficiency during centrifugation.