1. What is Relative Centrifugal Force?

Relative Centrifugal Force (RCF) is the measurement of the force applied to a sample within a centrifuge. It is expressed in multiples of the earth's gravitational force (g) and provides a standardized way to compare centrifugation protocols across different equipment.

2. How Does the Calculator Work?

The calculator uses the RCF formula:

Where:

• \( RCF \) — Relative centrifugal force (g)
• \( r \) — Rotor radius in centimeters (cm)
• \( RPM \) — Rotations per minute (rev/min)
• \( 1.118 \times 10^{-5} \) — Conversion constant

Explanation: The formula converts rotational speed and radius into gravitational equivalents, allowing for standardized centrifugation protocols.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is essential for reproducible laboratory results, proper sample separation, and ensuring experimental consistency across different centrifuges and laboratories.

4. Using the Calculator

Tips: Enter the rotor radius in centimeters and the rotational speed in RPM. Both values must be positive numbers. The radius is measured from the center of the rotor to the sample.

5. Frequently Asked Questions (FAQ)

Q1: Why use RCF instead of RPM?
A: RCF provides a standardized measurement that accounts for different rotor sizes, ensuring consistent separation results across various centrifuge models.

Q2: How do I measure rotor radius?
A: Measure from the center of the rotor to the middle of the sample tube when it's in the operating position.

Q3: What are typical RCF values used in laboratories?
A: RCF values range from 100g for gentle separations to over 100,000g for high-speed ultracentrifugation applications.

Q4: Can I convert RCF back to RPM?
A: Yes, the formula can be rearranged: \( RPM = \sqrt{\frac{RCF}{1.118 \times 10^{-5} \times r}} \)

Q5: Why is the constant 1.118 × 10^{-5} used?
A: This constant incorporates the conversion factors for units (cm to m, minutes to seconds) and the gravitational acceleration constant.