1. What is RCF?

Relative Centrifugal Force (RCF), also known as g-force, is the measurement of the gravitational force applied in a centrifuge. It provides a standardized way to compare centrifugation conditions across different equipment.

2. How Does the Calculator Work?

The calculator uses the RCF equation at 3000 RPM:

Where:

• \( RCF \) — Relative Centrifugal Force (g)
• \( r \) — Radius in centimeters (cm)
• \( 1.118 \times 10^{-5} \) — Conversion constant
• \( 3000 \) — Rotations per minute (RPM)

Explanation: This equation converts rotational speed (RPM) to gravitational force (g) based on the radius of the centrifuge rotor.

3. Importance of RCF Calculation

Details: Accurate RCF calculation is crucial for reproducible laboratory results, proper sample separation, and ensuring consistent experimental conditions across different centrifuges.

4. Using the Calculator

Tips: Enter the radius in centimeters from the center of rotation to the sample. The radius must be greater than zero for accurate calculation.

5. Frequently Asked Questions (FAQ)

Q1: What is the difference between RPM and RCF?
A: RPM measures rotational speed, while RCF measures the actual gravitational force applied to samples. RCF is more meaningful for experimental reproducibility.

Q2: Why is radius important in RCF calculation?
A: Centrifugal force increases with radius at the same RPM. Samples at larger radii experience higher g-forces than those closer to the center.

Q3: What are typical RCF values used in laboratories?
A: RCF values range from 100-20,000 g depending on the application, with lower forces for cell separation and higher forces for pelleting small particles.

Q4: How do I measure the radius accurately?
A: Measure from the center of the rotor to the middle of the sample tube when it's in the horizontal position during rotation.

Q5: Can I use this calculator for different RPM values?
A: This calculator is specifically designed for 3000 RPM. For other speeds, use the general formula: RCF = 1.118 × 10^{-5} × r × RPM².